Patrik Edén
Universitetslektor
Microarray-based classification of a consecutive series of 121 childhood acute leukemias: prediction of leukemic and genetic subtype as well as of minimal residual disease status.
Författare
Summary, in English
Gene expression analyses were performed on 121 consecutive childhood leukemias (87 B-lineage acute lymphoblastic leukemias (ALLs), 11 T-cell ALLs and 23 acute myeloid leukemias (AMLs)), investigated during an 8-year period at a single center. The supervised learning algorithm k-nearest neighbor was utilized to build gene expression predictors that could classify the ALLs/AMLs according to clinically important subtypes with high accuracy. Validation experiments in an independent data set verified the high prediction accuracies of our classifiers. B-lineage ALLs with uncharacteristic cytogenetic aberrations or with a normal karyotype displayed heterogeneous gene expression profiles, resulting in low prediction accuracies. Minimal residual disease status (MRD) in T-cell ALLs with a high (40.1%) MRD at day 29 could be classified with 100% accuracy already at the time of diagnosis. In pediatric leukemias with uncharacteristic cytogenetic aberrations or with a normal karyotype, unsupervised analysis identified two novel subgroups: one consisting mainly of cases remaining in complete remission (CR) and one containing a few patients in CR and all but one of the patients who relapsed. This study of a consecutive series of childhood leukemias confirms and extends further previous reports demonstrating that global gene expression profiling provides a valuable tool for genetic and clinical classification of childhood leukemias.
Avdelning/ar
- Avdelningen för klinisk genetik
- Beräkningsbiologi och biologisk fysik - Har omorganiserats
- Pediatrik, Lund
- Avdelningen för hematologi och transfusionsmedicin
- Matematik LTH
- MLL-rearrangerad leukemi hos spädbarn
Publiceringsår
2007
Språk
Engelska
Sidor
1198-1203
Publikation/Tidskrift/Serie
Leukemia
Volym
21
Issue
6
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Nature Publishing Group
Ämne
- Cancer and Oncology
Nyckelord
- gene expression profiling
- pediatric leukemia
- supervised
- classification
- ALL
- AML
Status
Published
Forskningsgrupp
- The pathogenetic mechanisms behind MLL-rearranged acute leukemia in infancy
ISBN/ISSN/Övrigt
- ISSN: 1476-5551