Tobias Ambjörnsson
Universitetslektor
CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS
Författare
Summary, in English
We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a broad range of beta-lactam antibiotics.
Avdelning/ar
- Beräkningsbiologi och biologisk fysik - Har omorganiserats
- Institutionen för astronomi och teoretisk fysik - Har omorganiserats
Publiceringsår
2021
Språk
Engelska
Sidor
801-802
Publikation/Tidskrift/Serie
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Dokumenttyp
Konferensbidrag
Förlag
Chemical and Biological Microsystems Society
Ämne
- Microbiology
Nyckelord
- Anti-microbial resistance
- CRISPR/Cas9
- DNA
- DNA combing
- Plasmid
- Single-molecule
Conference name
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Conference date
2021-10-10 - 2021-10-14
Conference place
Palm Springs, Virtual, United States
Status
Published
ISBN/ISSN/Övrigt
- ISBN: 9781733419031