Tobias Ambjörnsson
Universitetslektor
Rapid tracing of resistance plasmids in a nosocomial outbreak using optical DNA mapping
Författare
Summary, in English
Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum β-lactamases (ESBL) and carbapenemases on highly mobile plasmids. We here demonstrate how optical DNA maps of single plasmids can be used as fingerprints to trace plasmids, for example, during resistance outbreaks. We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). Our results demonstrate that optical DNA mapping is an easy and rapid method for detecting the spread of plasmids mediating resistance. With the increasing prevalence of multiresistant bacteria, diagnostic tools that can aid in solving ongoing routes of transmission, in particular in hospital settings, will be of paramount importance.
Avdelning/ar
- Beräkningsbiologi och biologisk fysik - Har omorganiserats
Publiceringsår
2016-05-13
Språk
Engelska
Sidor
322-328
Publikation/Tidskrift/Serie
ACS Infectious Diseases
Volym
2
Issue
5
Dokumenttyp
Artikel i tidskrift
Förlag
The American Chemical Society (ACS)
Ämne
- Medicinal Chemistry
- Infectious Medicine
- Biophysics
- Other Physics Topics
Nyckelord
- antibiotic resistance
- nosocomial outbreak
- optical DNA mapping
- plasmids
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 2373-8227