Tobias Ambjörnsson
Universitetslektor
Bacterial identification by optical mapping of genomic DNA in nanofluidic channels
Författare
Summary, in English
A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.
Avdelning/ar
- Beräkningsbiologi och biologisk fysik - Har omorganiserats
Publiceringsår
2019
Språk
Engelska
Sidor
821-822
Publikation/Tidskrift/Serie
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Dokumenttyp
Konferensbidrag
Förlag
Chemical and Biological Microsystems Society
Ämne
- Microbiology in the medical area
- Infectious Medicine
- Biophysics
- Other Physics Topics
Nyckelord
- Bacterial Identification
- Nanofluidics
- Optical DNA Mapping
Conference name
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Conference date
2019-10-27 - 2019-10-31
Conference place
Basel, Switzerland
Status
Published
ISBN/ISSN/Övrigt
- ISBN: 9781733419000