Cryo-EM Reveals Promoter DNA Binding and Conformational Flexibility of the General Transcription Factor TFIID

Summary, in English

The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the THID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.

• Författare: Hans Elmlund; Vera Baraznenok; Tomas Linder; Zsolt Szilagyi; Reza Rofougaran; Anders Hofer; Hans Hebert; Martin Lindahl; Claes M. Gustafsson
• Avdelning/ar: Biokemi och Strukturbiologi
• Publiceringsår: 2009
• Språk: Engelska
• Sidor: 1442-1452
• Publikation/Tidskrift/Serie: Structure
• Volym: 17
• Issue: 11
• Länkar: http://dx.doi.org/10.1016/j.str.2009.09.007
• Dokumenttyp: Artikel i tidskrift
• Förlag: Cell Press
• Ämne: Biological Sciences
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 0969-2126