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Maria Hansson. Foto.

Maria Hansson

Universitetslektor

Maria Hansson. Foto.

An evaluation of DNA extraction methods on historical and roadkill mammalian specimen

Författare

  • Noëlie Molbert
  • Hamid Reza Ghanavi
  • Tomas Johansson
  • Maria Mostadius
  • Maria C Hansson

Summary, in English

Guidelines identifying appropriate DNA extraction methods for both museum and modern biological samples are scarce or non-existent for mammalian species. Yet, obtaining large-scale genetic material collections are vital for conservation and management purposes. In this study, we evaluated five protocols making use of either spin-column, organic solvents, or magnetic bead-based methods for DNA extraction on skin samples from both modern, traffic-killed (n = 10) and museum (n = 10) samples of European hedgehogs, Ericaneus europaeus. We showed that phenol-chloroform or silica column (NucleoSpin Tissue) protocols yielded the highest amount of DNA with satisfactory purity compared with magnetic bead-based protocols, especially for museum samples. Furthermore, extractions using the silica column protocol appeared to produce longer DNA fragments on average than the other methods tested. Our investigation demonstrates that both commercial extraction kits and phenol-chloroform protocol retrieve acceptable DNA concentrations for downstream processes, from degraded remnants of traffic-killed and museum samples of mammalian specimens. Although all the tested methods could be applied depending on the research questions and laboratory conditions, commercial extraction kits may be preferred due to their effectiveness, safety and the higher quality of the DNA extractions.

Avdelning/ar

  • Centrum för miljö- och klimatvetenskap (CEC)
  • Funktionell zoologi
  • Biodiversitet
  • Molekylär ekologi och evolution
  • Mikrobiologisk ekologi
  • MEMEG
  • Biologiska museet
  • Zoologiska samlingarna
  • BECC: Biodiversity and Ecosystem services in a Changing Climate

Publiceringsår

2023-08-11

Språk

Engelska

Publikation/Tidskrift/Serie

Scientific Reports

Volym

13

Dokumenttyp

Artikel i tidskrift

Förlag

Nature Publishing Group

Ämne

  • Zoology
  • Genetics

Nyckelord

  • Animals
  • Chloroform
  • DNA/genetics
  • Phenol
  • Phenols
  • Mammals/genetics
  • Silicon Dioxide

Status

Published

Forskningsgrupp

  • Molecular Ecology and Evolution Lab
  • Microbial Ecology

ISBN/ISSN/Övrigt

  • ISSN: 2045-2322