Quantification of growth and nutrient consumption of bacterial and fungal cultures in microfluidic microhabitat models

Summary, in English

Understanding microbes in nature requires consideration of their microenvironment. Here, we present a protocol for quantifying biomass and nutrient degradation of bacterial and fungal cultures (Pseudomonas putida and Coprinopsis cinerea, respectively) in microfluidics. We describe steps for mask design and fabrication, master printing, polydimethylsiloxane chip fabrication, and chip inoculation and imaging using fluorescence microscopy. We include procedures for image analysis, plotting, and statistics. For complete details on the use and execution of this protocol, please refer to Arellano-Caicedo et al. (2023).¹

• Författare: Carlos Arellano-Caicedo; Jason P. Beech; Martin Bengtsson; Pelle Ohlsson; Edith C. Hammer
• Avdelning/ar: Biologiska institutionen; Fasta tillståndets fysik; Avdelningen för biomedicinsk teknik; BECC: Biodiversity and Ecosystem services in a Changing Climate; NanoLund: Centre for Nanoscience; LU profilområde: Naturbaserade framtidslösningar
• Publiceringsår: 2024-03-15
• Språk: Engelska
• Publikation/Tidskrift/Serie: STAR Protocols
• Volym: 5
• Issue: 1
• Länkar: http://dx.doi.org/10.1016/j.xpro.2023.102784
• Dokumenttyp: Artikel i tidskrift
• Förlag: Cell Press
• Ämne: Microbiology
• Nyckelord: Environmental sciences; Microbiology; Microscopy
• Aktiv: Published
• ISBN/ISSN/Övrigt: ISSN: 2666-1667