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Carsten Peterson

Expert

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Elucidating fungal decomposition of organic matter at sub-micrometer spatial scales using optical photothermal infrared (O-PTIR) microspectroscopy

Författare

  • Michiel Op De Beeck
  • Carl Troein
  • Carsten Peterson
  • Anders Tunlid
  • Per Persson

Summary, in English

In microbiological studies, a common goal is to link environmental factors to microbial activities. Both environmental factors and microbial activities are typically derived from bulk samples. It is becoming increasingly clear that such bulk environmental parameters poorly represent the microscale environments microorganisms experience. Using infrared (IR) microspectroscopy, the spatial distribution of chemical compound classes can be visualized, making it a useful tool for studying the interactions between microbial cells and their microenvironments. The spatial resolution of conventional IR microspectroscopy has been limited by the diffractionlimit of IR light. The recent development of optical photothermal infrared (O-PTIR) microspectroscopy has pushed the spatial resolution of IR microspectroscopy beyond this diffractionlimit, allowing the distribution of chemical compound classes to be visualized at sub-micrometer spatial scales. To examine the potential and limitations of O-PTIR microspectroscopy to probe the interactions between fungal cells and their immediate environments, we imaged the decomposition of cellulose filmsby cells of the ectomycorrhizal fungus Paxillus involutus and compared O-PTIR results using conventional IR microspectroscopy. Whereas the data collected with conventional IR microspectroscopy indicated that P. involutus has only a very limited ability to decompose cellulose films,O-PTIR data suggested that the ability of P. involutus to decompose cellulose was substantial. Moreover, the O-PTIR method enabled the identificationof a zone located outside the fungal hyphae where the cellulose was decomposed by oxidation. We conclude that O-PTIR can provide valuable new insights into the abilities and mechanisms by which microorganisms interact with their surrounding environments.

Avdelning/ar

  • Centrum för miljö- och klimatvetenskap (CEC)
  • BECC: Biodiversity and Ecosystem services in a Changing Climate
  • Beräkningsvetenskap för hälsa och miljö
  • MEMEG
  • Mikrobiologisk ekologi

Publiceringsår

2024-02

Språk

Engelska

Publikation/Tidskrift/Serie

Applied and Environmental Microbiology

Volym

90

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

American Society for Microbiology

Ämne

  • Soil Science

Nyckelord

  • cellulose
  • decomposition
  • infrared microspectroscopy
  • microenvironment
  • microorganism
  • organic matter

Aktiv

Published

Forskningsgrupp

  • Computational Science for Health and Environment
  • Microbial Ecology

ISBN/ISSN/Övrigt

  • ISSN: 0099-2240