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Carsten Peterson

Expert

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Fenton reaction facilitates organic nitrogen acquisition by an ectomycorrhizal fungus

Författare

  • Michiel Op De Beeck
  • Carl Troein
  • Carsten Peterson
  • Per Persson
  • Anders Tunlid

Summary, in English

Boreal trees rely on their ectomycorrhizal fungal symbionts to acquire growth-limiting nutrients, such as nitrogen (N), which mainly occurs as proteins complexed in soil organic matter (SOM). The mechanisms for liberating this N are unclear as ectomycorrhizal fungi have lost many genes encoding lignocellulose-degrading enzymes present in their saprotrophic ancestors. We hypothesized that hydroxyl radicals (bullOH), produced by the ectomycorrhizal fungus Paxillus involutus during growth on SOM, are involved in liberating organic N. Paxillus involutus was grown for 7 d on N-containing or N-free substrates that represent major organic compounds of SOM. bullOH production, ammonium assimilation, and proteolytic activity were measured daily. bullOH production was strongly induced when P. involutus switched from ammonium to protein as the main N source. Extracellular proteolytic activity was initiated shortly after the oxidation. Oxidized protein substrates induced higher proteolytic activity than unmodified proteins. Dynamic modeling predicted that bullOH production occurs in a burst, regulated mainly by ammonium and ferric iron concentrations. We propose that the production of bullOH and extracellular proteolytic enzymes are regulated by similar nutritional signals. Oxidation works in concert with proteolysis, improving N liberation from proteins in SOM. Organic N mining by ectomycorrhizal fungi has, until now, only been attributed to proteolysis.

Avdelning/ar

  • MEMEG
  • Computational Biology and Biological Physics - Has been reorganised
  • Centrum för miljö- och klimatvetenskap (CEC)
  • BECC: Biodiversity and Ecosystem services in a Changing Climate
  • Mikrobiologisk ekologi

Publiceringsår

2018-01-03

Språk

Engelska

Sidor

335-343

Publikation/Tidskrift/Serie

New Phytologist

Volym

218

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Microbiology
  • Bioinformatics and Systems Biology
  • Botany
  • Other Physics Topics

Nyckelord

  • Fenton reaction
  • Nitrogen (N)
  • Paxillus involutus
  • Proteolysis
  • Soil organic matter (SOM)

Aktiv

Published

Projekt

  • MICCS - Molecular Interactions Controlling soil Carbon Sequestration

Forskningsgrupp

  • Microbial Ecology

ISBN/ISSN/Övrigt

  • ISSN: 0028-646X