Bacterial identification by optical mapping of genomic DNA in nanofluidic channels

Summary, in English

A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.

• Author: My Nyblom; Vilhelm Müller; Anna Johnning; Marie Wrande; Albertas Dvirnas; K. K. Sriram; Christian G. Giske; Tobias Ambjörnsson; Linus Sandegren; Erik Kristiansson; Fredrik Westerlund
• Department/s: Computational Biology and Biological Physics - Has been reorganised
• Publishing year: 2019
• Language: English
• Pages: 821-822
• Publication/Series: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
• Document type: Conference paper
• Publisher: Chemical and Biological Microsystems Society
• Topic: Microbiology in the medical area; Infectious Medicine; Biophysics; Other Physics Topics
• Keywords: Bacterial Identification; Nanofluidics; Optical DNA Mapping
• Conference name: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
• Conference date: 2019-10-27 - 2019-10-31
• Conference place: Basel, Switzerland
• Status: Published
• ISBN/ISSN/Other: ISBN: 9781733419000