
Patrik Edén
Senior lecturer

Identification of uniquely expressed transcription factors in highly purified B-cell lymphoma samples.
Author
Summary, in English
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repression and transcriptional activation. Still, this information is not used for classification of B-cell lymphomas (BCLs). Traditionally, BCLs are diagnosed based on a phenotypic resemblance to normal B-cells; assessed by immunohistochemistry or flow cytometry, by using a handful of phenotypic markers. In the last decade, diagnostic and prognostic evaluation has been facilitated by global gene expression profiling (GEP), providing a new powerful means for the classification, prediction of survival, and response to treatment of lymphomas. However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. In this study, global GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treatment.
Department/s
- Department of Immunotechnology
- Computational Biology and Biological Physics - Undergoing reorganization
- Breastcancer-genetics
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publishing year
2010
Language
English
Pages
418-425
Publication/Series
American Journal of Hematology
Volume
85
Issue
6
Document type
Journal article
Publisher
John Wiley & Sons Inc.
Topic
- Hematology
Keywords
- B-Cell: genetics
- Lymphoma
- B-Cell: pathology
- Neoplasm Proteins: biosynthesis
- Neoplasm Proteins: genetics
- Messenger: biosynthesis
- RNA
- Neoplasm: biosynthesis
- Transcription Factors: genetics
- Transcription Factors: biosynthesis
- B-Lymphocyte Subsets: pathology
- B-Lymphocyte Subsets: metabolism
- B-Cell: classification
Status
Published
ISBN/ISSN/Other
- ISSN: 0361-8609