Martin Lindahl
Programmer
The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II
Author
Summary, in English
CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.
Department/s
- Biochemistry and Structural Biology
Publishing year
2006
Language
English
Pages
15788-15793
Publication/Series
Proceedings of the National Academy of Sciences
Volume
103
Issue
43
Document type
Journal article
Publisher
National Academy of Sciences
Topic
- Biological Sciences
Keywords
- protein structure
- electron microscopy
- transcription
- Schizosaccharomyces pombe
- yeast
Status
Published
ISBN/ISSN/Other
- ISSN: 1091-6490