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Martin Lindahl. Photo.

Martin Lindahl

Programmer

Martin Lindahl. Photo.

The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II

Author

  • Hans Elmlund
  • Vera Baraznenok
  • Martin Lindahl
  • Camilla O. Samuelsen
  • Philip J. B. Koeck
  • Steen Holmberg
  • Hans Hebert
  • Claes M. Gustafsson

Summary, in English

CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.

Department/s

  • Biochemistry and Structural Biology

Publishing year

2006

Language

English

Pages

15788-15793

Publication/Series

Proceedings of the National Academy of Sciences

Volume

103

Issue

43

Document type

Journal article

Publisher

National Academy of Sciences

Topic

  • Biological Sciences

Keywords

  • protein structure
  • electron microscopy
  • transcription
  • Schizosaccharomyces pombe
  • yeast

Status

Published

ISBN/ISSN/Other

  • ISSN: 1091-6490