Edith Hammer
Senior lecturer
Quantification of growth and nutrient consumption of bacterial and fungal cultures in microfluidic microhabitat models
Author
Summary, in English
Understanding microbes in nature requires consideration of their microenvironment. Here, we present a protocol for quantifying biomass and nutrient degradation of bacterial and fungal cultures (Pseudomonas putida and Coprinopsis cinerea, respectively) in microfluidics. We describe steps for mask design and fabrication, master printing, polydimethylsiloxane chip fabrication, and chip inoculation and imaging using fluorescence microscopy. We include procedures for image analysis, plotting, and statistics. For complete details on the use and execution of this protocol, please refer to Arellano-Caicedo et al. (2023).1
Department/s
- Department of Biology
- Solid State Physics
- Department of Biomedical Engineering
Publishing year
2024-03-15
Language
English
Publication/Series
STAR Protocols
Volume
5
Issue
1
Document type
Journal article
Publisher
Cell Press
Topic
- Microbiology
Keywords
- Environmental sciences
- Microbiology
- Microscopy
Status
Published
ISBN/ISSN/Other
- ISSN: 2666-1667