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Edith Hammer. Photo.

Edith Hammer

Senior lecturer

Edith Hammer. Photo.

Quantification of growth and nutrient consumption of bacterial and fungal cultures in microfluidic microhabitat models

Author

  • Carlos Arellano-Caicedo
  • Jason P. Beech
  • Martin Bengtsson
  • Pelle Ohlsson
  • Edith C. Hammer

Summary, in English

Understanding microbes in nature requires consideration of their microenvironment. Here, we present a protocol for quantifying biomass and nutrient degradation of bacterial and fungal cultures (Pseudomonas putida and Coprinopsis cinerea, respectively) in microfluidics. We describe steps for mask design and fabrication, master printing, polydimethylsiloxane chip fabrication, and chip inoculation and imaging using fluorescence microscopy. We include procedures for image analysis, plotting, and statistics. For complete details on the use and execution of this protocol, please refer to Arellano-Caicedo et al. (2023).1

Department/s

  • Department of Biology
  • Solid State Physics
  • Division for Biomedical Engineering
  • BECC: Biodiversity and Ecosystem services in a Changing Climate
  • NanoLund: Centre for Nanoscience
  • LU Profile Area: Nature-based future solutions

Publishing year

2024-03-15

Language

English

Publication/Series

STAR Protocols

Volume

5

Issue

1

Document type

Journal article

Publisher

Cell Press

Topic

  • Microbiology

Keywords

  • Environmental sciences
  • Microbiology
  • Microscopy

Status

Published

ISBN/ISSN/Other

  • ISSN: 2666-1667