The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

rhiannon standing in autumn forest

Rhiannon Mondav

Postdoc

rhiannon standing in autumn forest

Modified Nucleic Acid Extraction Protocol for Humic-Rich Permafrost Peat Samples v1

Author

  • Yueh-Fen Li
  • Rhiannon Mondav
  • Virginia Rich

Summary, in English

Slight modifications to the manufacturer’s protocol (Qiagen #12966-10, now discontinued and replaced with #12988-10) enabled the successful extraction of high-quality DNA and RNA from permafrost peat samples. Integrating the DNA and RNA purification steps immediately after the extraction process helped streamline the workflow, resulting in consistent quality nucleic acids suitable for downstream sequencing and analysis. This improved approach optimizes the extraction process, ensuring more reliable and efficient results for permafrost peat studies.

Department/s

  • Centre for Environmental and Climate Science (CEC)
  • BECC: Biodiversity and Ecosystem services in a Changing Climate

Publishing year

2024-11-01

Language

English

Document type

Preprint

Publisher

Springer Nature

Topic

  • Microbiology
  • Genetics and Genomics
  • Biological Sciences

Keywords

  • DNA/*analysis/isolation & purification
  • RNA analysis
  • peat

Status

Published