Tobias Ambjörnsson
Universitetslektor
Identifying bacteria using DNA binding maps
Författare
Summary, in English
We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.
Avdelning/ar
- Synkrotronljusfysik
- Beräkningsbiologi och biologisk fysik - Har omorganiserats
- Fasta tillståndets fysik
Publiceringsår
2013-01-01
Språk
Engelska
Sidor
473-475
Publikation/Tidskrift/Serie
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volym
1
Dokumenttyp
Konferensbidrag
Förlag
Chemical and Biological Microsystems Society
Ämne
- Biophysics
- Other Physics Topics
Nyckelord
- Bacterial identification
- Nanofluidics
- Optical mapping
Conference name
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Conference date
2013-10-27 - 2013-10-31
Conference place
Freiburg, Germany
Status
Published
ISBN/ISSN/Övrigt
- ISBN: 9781632666246