Peter Olsson
Researcher
Kinetics of the coupled reaction catalysed by a fusion protein of yeast mitochondrial malate dehydrogenase and citrate synthase: Kinetics of a fusion protein of MDH and CS
Author
Summary, in English
The mechanistic implications of the kinetic behaviour of a fusion protein of mitochondrial malate dehydrogenase and citrate synthase have been reanalysed in view of predictions based on experimentally determined kinetic parameter values for the dehydrogenase and synthase activities of the protein. The results show that the time-course of citrate formation from malate in the coupled reaction catalysed by the fusion protein can be most satisfactorily accounted for in terms of a free-diffusion mechanism when consideration is taken to the inhibitory effects of NADH and oxaloacetate on the malate dehydrogenase activity. The effect of aspartate aminotransferase on the coupled reaction is likewise fully consistent with that expected for a free-diffusion mechanism. It is concluded that no tenable kinetic evidence is available to support the proposal that the fusion protein catalyses citrate formation from malate by a mechanism involving channelling of the intermediate oxaloacetate.
Department/s
- Biochemistry and Structural Biology
- Pure and Applied Biochemistry
Publishing year
2000
Language
English
Pages
5041-5046
Publication/Series
European Journal of Biochemistry
Volume
267
Issue
16
Links
Document type
Journal article
Publisher
Wiley-Blackwell
Topic
- Theoretical Chemistry
- Biochemistry and Molecular Biology
Keywords
- fusion protein
- channelling
- malate dehydrogenase
- Citrate (si)-Synthase
- Oxaloacetic Acid
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- NAD
- Models, Chemical
- Mitochondria
- Kinetics
Status
Published
ISBN/ISSN/Other
- ISSN: 0014-2956