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Photo of Victor Olariu

Victor Olariu

Senior lecturer

Photo of Victor Olariu

Modeling the evolution of culture-adapted human embryonic stem cells

Author

  • Victor Olariu
  • Neil J. Harrison
  • Daniel Coca
  • Paul J. Gokhale
  • Duncan Baker
  • Steve Billings
  • Visakan Kadirkamanathan
  • Peter W. Andrews

Summary, in English

The long-term culture of human embryonic stem (ES) cells is inevitably subject to evolution, since any mutant that arises with a growth advantage will be selectively amplified. However, the evolutionary influences of population size, mutation rate, and selection pressure are frequently overlooked. We have constructed a Monte Carlo simulation model to predict how changes in these factors can influence the appearance and spread of mutant ES cells, and verified its applicability by comparison with in vitro data. This simulation provides an estimate for the expected rate of generation of culture-adapted ES cells under different assumptions for the key parameters. In particular, it highlights the effect of population size, suggesting that the maintenance of cells in small populations reduces the likelihood that abnormal cultures will develop.

Publishing year

2010-01

Language

English

Pages

50-56

Publication/Series

Stem Cell Research

Volume

4

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Cell and Molecular Biology
  • Biophysics

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-5061