CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS

Summary, in English

We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a broad range of beta-lactam antibiotics.

• Author: Gaurav Goyal; Elina Ekedahl; My Nyblom; Jens Krog; Erik Torstensson; Tsegaye Sewunet; Christian Giske; Visanu Thamlikitkul; Tobias Ambjörnsson; Fredrik Westerlund
• Department/s: Computational Biology and Biological Physics - Undergoing reorganization; Department of Astronomy and Theoretical Physics - Undergoing reorganization
• Publishing year: 2021
• Language: English
• Pages: 801-802
• Publication/Series: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
• Document type: Conference paper
• Publisher: Chemical and Biological Microsystems Society
• Topic: Microbiology
• Keywords: Anti-microbial resistance; CRISPR/Cas9; DNA; DNA combing; Plasmid; Single-molecule
• Conference name: 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
• Conference date: 2021-10-10 - 2021-10-14
• Conference place: Palm Springs, Virtual, United States
• Status: Published
• ISBN/ISSN/Other: ISBN: 9781733419031