Tobias Ambjörnsson
Senior lecturer
CRISPR/CAS9 BASED DNA-COMBING ASSAY FOR DETECTING ANTIMICROBIAL RESISTANCE GENES ON PLASMIDS
Author
Summary, in English
We present a method based on CRISPR/Cas9 excision and DNA combing to detect anti-microbial resistance (AMR) genes on bacterial plasmids. The assay is inexpensive, simple, fast, and also provides information on the number and size of plasmids in a sample. We demonstrate detection of the gene encoding for the New Delhi metallobeta-lactamase 1 (blaNDM-1) enzyme, known to make bacteria resistant to a broad range of beta-lactam antibiotics.
Department/s
- Computational Biology and Biological Physics - Undergoing reorganization
- Department of Astronomy and Theoretical Physics - Undergoing reorganization
Publishing year
2021
Language
English
Pages
801-802
Publication/Series
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Document type
Conference paper
Publisher
Chemical and Biological Microsystems Society
Topic
- Microbiology
Keywords
- Anti-microbial resistance
- CRISPR/Cas9
- DNA
- DNA combing
- Plasmid
- Single-molecule
Conference name
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Conference date
2021-10-10 - 2021-10-14
Conference place
Palm Springs, Virtual, United States
Status
Published
ISBN/ISSN/Other
- ISBN: 9781733419031