Tobias Ambjörnsson
Universitetslektor
Detailed characterization of plasmids carrying resistance genes using optical DNA mapping
Författare
Summary, in English
We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.
Avdelning/ar
- Beräkningsbiologi och biologisk fysik - Har omorganiserats
Publiceringsår
2016
Språk
Engelska
Sidor
1561-1562
Publikation/Tidskrift/Serie
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Dokumenttyp
Konferensbidrag
Förlag
Chemical and Biological Microsystems Society
Ämne
- Medical Laboratory and Measurements Technologies
- Biophysics
- Other Physics Topics
Nyckelord
- Antibiotic resistance
- CRISPR/Cas9
- Nanofluidics
- Optical DNA mapping
- Plasmids
Conference name
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Conference date
2016-10-09 - 2016-10-13
Conference place
Dublin, Ireland
Status
Published
ISBN/ISSN/Övrigt
- ISBN: 9780979806490