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Porträttbild av Peter Olsson. Foto.

Peter Olsson

Forskare

Porträttbild av Peter Olsson. Foto.

An oat branmeal influences blood insulin levels and related gene sets in peripheral blood mononuclear cells of healthy subjects

Författare

  • Matilda Ulmius
  • Anna Johansson Persson
  • Morten Krogh
  • Peter Olsson
  • Gunilla Önning

Summary, in English

The understanding of how fibre-rich meals regulate molecular events at a gene level is limited. This pilot study aimed to investigate changes in gene expression in peripheral blood mononuclear cells (PBMCs) from healthy subjects after consumption of an oat bran-rich meal. Fifteen subjects (8 men and 7 women, aged 20-28 years) ingested meals with oat bran or a control meal after an overnight fast. Blood samples for analysis of postprandial glucose, insulin and triglyceride concentrations were taken during 3 h, while PBMCs for microarray gene expression profiling from five men and five women were taken before and 2 h after the meal. Analysis of transcriptome data was performed with linear mixed models to determine differentially expressed genes in response either to meal intake or meal content, and enrichment analysis was used to identify functional gene sets responding to meal intake and specifically to oat bran intake. Meal intake as such affected gene expression for genes mainly involved in metabolic stress; indicating increased inflammation due to the switch from fasting to fed state. The oat bran meal affected gene sets associated with a lower insulin level, compared with the control meal. The gene sets included genes involved in insulin secretion and β-cell development, but also protein synthesis and genes related to cancer diseases. The oat bran meal also significantly lowered postprandial blood insulin IAUC compared to control. Further studies are needed to compare these acute effects with the long-term health effects of oat bran.

Avdelning/ar

  • Tillämpad biokemi
  • Beräkningsbiologi och biologisk fysik - Har omorganiserats

Publiceringsår

2011

Språk

Engelska

Sidor

429-439

Publikation/Tidskrift/Serie

Genes & Nutrition

Volym

6

Issue

4

Dokumenttyp

Artikel i tidskrift

Förlag

BioMed Central (BMC)

Ämne

  • Biochemistry and Molecular Biology

Nyckelord

  • Oats Microarray gene expression Peripheral mononuclear blood cells Postprandial response

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1555-8932