Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Milda Pucetaite. Foto.

Milda Pucetaite


Milda Pucetaite. Foto.

Assignment of vibrational spectral bands of kidney tissue by means of low temperature SERS spectroscopy


  • M. Velicka
  • M. Radzvilaite
  • Justinas Ceponkus
  • V. Urboniene
  • M. Pucetaite
  • F. Jankevicius
  • Gerald Steiner
  • V. Sablinskas


  • Daniel L. Farkas
  • Dan V. Nicolau
  • Robert C. Leif

Summary, in English

Surface enhanced Raman scattering (SERS) spectroscopy is a useful method for detection of trace amounts of molecules. It has already been successfully implemented for detection of explosives, food additives, biomarkers in blood or urine, etc. In the last decade, SERS spectroscopy was introduced into the field of health sciences and has been especially focused on early disease detection. In the recent years, application of SERS spectroscopy for detection of various types of human cancerous tissues emerged. Furthermore, SERS spectroscopy of extracellular fluid shows great potential for the differentiation of normal and cancerous tissues; however, due to high variety of molecules present in such biological samples, the experimental spectrum is a combination of many different overlapping vibrational spectral bands. Thus, precise assignment of these bands to the corresponding molecular vibrations is a difficult task. In most cases, researchers try to avoid this task satisfying just with tentative assignment. In this study, low temperature SERS measurements of extracellular fluid of cancerous and healthy kidney tissue samples were carried out in order to get a deeper understanding of the nature of vibrational spectral bands present in the experimental spectrum. The SERS spectra were measured in temperature range from 300 K down to 100 K. SERS method was implemented using silver nanoparticle colloidal solution. The results of the low temperature SERS experiment were analysed and compared with the results of theoretical calculations. The analysis showed that the SERS spectrum of extracellular fluid of kidney tissue is highly influenced by the vibrational bands of adenine and Lcystine molecules.






Proceedings of SPIE








  • Extracellular fluid
  • Kidney cancer
  • Low temperature
  • SERS

Conference name

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV

Conference date

2017-01-30 - 2017-02-01

Conference place

San Francisco, United States




  • ISSN: 0277-786X
  • ISSN: 1996-756X
  • ISBN: 9781510605770