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Milda Pucetaite. Foto.

Milda Pucetaite

Forskare

Milda Pucetaite. Foto.

Differentiation of bacterial strains by means of surface enhanced FT-Raman spectroscopy

Författare

  • K. Kairyte
  • Z. Luksiene
  • Milda Pucetaite
  • Valdas Sablinskas

Summary, in English

The silver nanoparticle colloid was used to obtain surface enhanced Raman spectra of Listeria monocytogenes, Salmonela enterica, and Esherichia coli bacteria. The SERS spectra were captured using for excitation the near-infrared (1064 nm) laser radiation with reduced intensity, which ensured the prevention of the fluorescence background as well as photo- and thermal decomposition of the samples. It was found that the optimal size of silver nanoparticles for the enhancement of the Raman signal in the near-infrared spectral region is ca. 50 nm. The spectral data obtained in this study indicate that relative intensities of SERS spectral bands of bacteria can be used for spectral differentiation of bacteria. In case of Listeria, Salmonela, and Esherichia cells, the intensity ratio of spectral bands of adenine and cysteine can be used as a spectral marker for differentiation of the bacteria.

Publiceringsår

2012

Språk

Engelska

Sidor

276-283

Publikation/Tidskrift/Serie

Lithuanian Journal of Physics

Volym

52

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

Lithuanian Physical Society

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1648-8504