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Differentiation of bacterial strains by means of surface enhanced FT-Raman spectroscopy

Author:
  • K. Kairyte
  • Z. Luksiene
  • Milda Pucetaite
  • Valdas Sablinskas
Publishing year: 2012
Language: English
Pages: 276-283
Publication/Series: Lithuanian Journal of Physics
Volume: 52
Issue: 3
Document type: Journal article
Publisher: Lithuanian Physical Society

Abstract english

The silver nanoparticle colloid was used to obtain surface enhanced Raman spectra of Listeria monocytogenes, Salmonela enterica, and Esherichia coli bacteria. The SERS spectra were captured using for excitation the near-infrared (1064 nm) laser radiation with reduced intensity, which ensured the prevention of the fluorescence background as well as photo- and thermal decomposition of the samples. It was found that the optimal size of silver nanoparticles for the enhancement of the Raman signal in the near-infrared spectral region is ca. 50 nm. The spectral data obtained in this study indicate that relative intensities of SERS spectral bands of bacteria can be used for spectral differentiation of bacteria. In case of Listeria, Salmonela, and Esherichia cells, the intensity ratio of spectral bands of adenine and cysteine can be used as a spectral marker for differentiation of the bacteria.

Other

Published
  • ISSN: 1648-8504
Milda Pucetaite. Photo.
E-mail: milda [dot] pucetaite [at] cec [dot] lu [dot] se

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