The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Milda Pucetaite. Photo.

Milda Pucetaite

Researcher

Milda Pucetaite. Photo.

Differentiation of bacterial strains by means of surface enhanced FT-Raman spectroscopy

Author

  • K. Kairyte
  • Z. Luksiene
  • Milda Pucetaite
  • Valdas Sablinskas

Summary, in English

The silver nanoparticle colloid was used to obtain surface enhanced Raman spectra of Listeria monocytogenes, Salmonela enterica, and Esherichia coli bacteria. The SERS spectra were captured using for excitation the near-infrared (1064 nm) laser radiation with reduced intensity, which ensured the prevention of the fluorescence background as well as photo- and thermal decomposition of the samples. It was found that the optimal size of silver nanoparticles for the enhancement of the Raman signal in the near-infrared spectral region is ca. 50 nm. The spectral data obtained in this study indicate that relative intensities of SERS spectral bands of bacteria can be used for spectral differentiation of bacteria. In case of Listeria, Salmonela, and Esherichia cells, the intensity ratio of spectral bands of adenine and cysteine can be used as a spectral marker for differentiation of the bacteria.

Publishing year

2012

Language

English

Pages

276-283

Publication/Series

Lithuanian Journal of Physics

Volume

52

Issue

3

Document type

Journal article

Publisher

Lithuanian Physical Society

Status

Published

ISBN/ISSN/Other

  • ISSN: 1648-8504