Mattias Ohlsson
Professor
Proteomic data analysis for differential profiling of the autoimmune diseases SLE, RA, SS, and ANCA-associated vasculitis
Author
Summary, in English
Early and correct diagnosis of inflammatory rheumatic diseases (IRD) poses a clinical challenge due to the multifaceted nature of symptoms, which also may change over time. The aim of this study was to perform protein expression profiling of four systemic IRDs, systemic lupus erythematosus (SLE), ANCA-associated systemic vasculitis (SV), rheumatoid arthritis (RA), and Sjögren's syndrome (SS), and healthy controls to identify candidate biomarker signatures for differential classification. A total of 316 serum samples collected from patients with SLE, RA, SS, or SV and from healthy controls were analyzed using 394-plex recombinant antibody microarrays. Differential protein expression profiling was examined using Wilcoxon signed rank test, and condensed biomarker panels were identified using advanced bioinformatics and state-of-the art classification algorithms to pinpoint signatures reflecting each disease (raw data set available at https:// figshare.com/s/3bd3848a28ef6e7ae9a9.). In this study, we were able to classify the included individual IRDs with high accuracy, as demonstrated by the ROC area under the curve (ROC AUC) values ranging between 0.96 and 0.80. In addition, the groups of IRDs could be separated from healthy controls at an ROC AUC value of 0.94. Disease-specific candidate biomarker signatures and general autoimmune signature were identified, including several deregulated analytes. This study supports the rationale of using multiplexed affinity-based technologies to reflect the biological complexity of autoimmune diseases. A multiplexed approach for decoding multifactorial complex diseases, such as autoimmune diseases, will play a significant role for future diagnostic purposes, essential to prevent severe organ- and tissue-related damage.
Department/s
- Computational Biology and Biological Physics - Has been reorganised
- eSSENCE: The e-Science Collaboration
- Autoimmunity and kidney diseases
- Lund SLE Research Group
- EpiHealth: Epidemiology for Health
- Rheumatology
- Department of Immunotechnology
Publishing year
2021
Language
English
Pages
1252-1260
Publication/Series
Journal of Proteome Research
Volume
20
Issue
2
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Rheumatology and Autoimmunity
Keywords
- Antibody microarray
- Autoimmune diseases
- Proteomics
- Whole blood
Status
Published
Research group
- Autoimmunity and kidney diseases
- Lund SLE Research Group
- Rheumatology
ISBN/ISSN/Other
- ISSN: 1535-3893