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Photo of Mattias Ohlsson

Mattias Ohlsson


Photo of Mattias Ohlsson

Proteomic data analysis for differential profiling of the autoimmune diseases SLE, RA, SS, and ANCA-associated vasculitis


  • Mattias Ohlsson
  • Thomas Hellmark
  • Anders A. Bengtsson
  • Elke Theander
  • Carl Turesson
  • Cecilia Klint
  • Christer Wingren
  • Anna Isinger Ekstrand

Summary, in English

Early and correct diagnosis of inflammatory rheumatic diseases (IRD) poses a clinical challenge due to the multifaceted nature of symptoms, which also may change over time. The aim of this study was to perform protein expression profiling of four systemic IRDs, systemic lupus erythematosus (SLE), ANCA-associated systemic vasculitis (SV), rheumatoid arthritis (RA), and Sjögren's syndrome (SS), and healthy controls to identify candidate biomarker signatures for differential classification. A total of 316 serum samples collected from patients with SLE, RA, SS, or SV and from healthy controls were analyzed using 394-plex recombinant antibody microarrays. Differential protein expression profiling was examined using Wilcoxon signed rank test, and condensed biomarker panels were identified using advanced bioinformatics and state-of-the art classification algorithms to pinpoint signatures reflecting each disease (raw data set available at https:// In this study, we were able to classify the included individual IRDs with high accuracy, as demonstrated by the ROC area under the curve (ROC AUC) values ranging between 0.96 and 0.80. In addition, the groups of IRDs could be separated from healthy controls at an ROC AUC value of 0.94. Disease-specific candidate biomarker signatures and general autoimmune signature were identified, including several deregulated analytes. This study supports the rationale of using multiplexed affinity-based technologies to reflect the biological complexity of autoimmune diseases. A multiplexed approach for decoding multifactorial complex diseases, such as autoimmune diseases, will play a significant role for future diagnostic purposes, essential to prevent severe organ- and tissue-related damage.


  • Computational Biology and Biological Physics - Undergoing reorganization
  • eSSENCE: The e-Science Collaboration
  • Autoimmunity and kidney diseases
  • Lund SLE Research Group
  • EpiHealth: Epidemiology for Health
  • Rheumatology
  • Department of Immunotechnology

Publishing year







Journal of Proteome Research





Document type

Journal article


The American Chemical Society (ACS)


  • Rheumatology and Autoimmunity


  • Antibody microarray
  • Autoimmune diseases
  • Proteomics
  • Whole blood



Research group

  • Autoimmunity and kidney diseases
  • Lund SLE Research Group
  • Rheumatology


  • ISSN: 1535-3893