Phosphorus Availability Influences Elemental Uptake in the Mycorrhizal Fungus Glomus intraradices, as Revealed by PIXE Analysis.

Summary, in English

We investigated element accumulation in the arbuscular mycorrhizal fungus Glomus intraradices. Fungal spores and mycelium growing in monoxenic cultures were analyzed. The elemental composition was quantified using particle-induced X-ray emission (PIXE) in combination with scanning transmission ion microscopy. In the spores, Ca and Fe were mainly associated with the spore wall; while P and K showed patchy distributions and their concentrations were correlated. Excess of P in the hyphal growth medium increased the P and Si concentration in spores, and increased the K:Ca ratio in spores. Increased P availability decreased the concentration of Zn and Mn in spores. We conclude that the availability of P influences the uptake and accumulation of several elements in spores. It is demonstrated that PIXE analysis is a powerful tool for quantitative analysis of elemental accumulation in fungal mycelia.

• Author: Pål Axel Olsson; Edith Hammer; Håkan Wallander; Jan Pallon
• Department/s: Biodiversity; MEMEG; Nuclear physics; Microbial Ecology; Biodiversity and Conservation Science; Plant Biology
• Publishing year: 2008
• Language: English
• Pages: 4144-4148
• Publication/Series: Applied and Environmental Microbiology
• Volume: 74
• Issue: 13
• Links: http://dx.doi.org/10.1128/AEM.00376-08
• Document type: Journal article
• Publisher: American Society for Microbiology
• Topic: Ecology; Subatomic Physics; Biological Sciences
• Status: Published
• Research group: Microbial Ecology; Biodiversity and Conservation Science; Plant Biology
• ISBN/ISSN/Other: ISSN: 0099-2240